P-003 Obstructive azoospermia; MESA should be employed as the method for sperm retrieval, not TESE

Abstract Study question TESE is widely used for obstructive azoospermia (OA) as a surgical method sperm retrieval, but it benefiting the patient? Summary answer Since MESA-ICSI has very good fertilization rate, clinical pregnancy rate and delivery MESA should be employed OA subjects, not TESE. What known already micro-TESE are technically simple, retrieval surgery because they do require microsurgical skills. Some review articles have shown no significant differences in rates of cleavage, good-quality embryos, implantation, between ICSI with epididymal or testicular sperm. Clinical usefulness still controversial according to previous reports. design, size, duration We studied 110 patients diagnosed treated at Asada Ladies Clinic April 2004 December 2021. Participants/materials, setting, methods The was performed using micropipette micropuncture technique under operative microscope. When were present motility observed, additional punctures tubule performed. Aspirated samples transferred into modified human tubal fluid sent vitro (IVF) laboratory cryopreservation. Main results role chance Motile recovered all cases (110 cases). Of these, frozen thawed 101 cases. normal 76%. 399 embryo transfer (ET) cycles, 168 had (41% per ET). who underwent ART, 94 (93% case) pregnancies resulting 90 (89.1%) deliveries. It emphasized that since does involve incision testes, there fewer postoperative peritoneal irritation symptoms concerns about atrophy low testosterone levels. showed embryo, Although controversial, if ART TESE-ICSI same, less invasive on patient reduces burden embryologist process tissue. Limitations, reasons caution authors reported specimens contain DNA fragmentated when compared specimen TESE, subsequent poorer rates. did evaluate fragmentation. Wider implications findings A large quantity uncontaminated can retrieved which patient, need special processing before Additionally, reduce workload. Trial registration number applicable